BeaverBeads™ Strep-Tag Ⅱ Protein Purification Beads
BeaverBeads™ Strep-Tag Ⅱ Protein Purification Beads
The Strep Tactin beads based on superparamagnetic beads are designed for rapid and efficient separation Strep（II）-tag protein. The Strep-tag is a synthetic peptide consisting of eight amino acids (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys). Generally, it does not interfere with folding or bioactivity. Thus, there is no need for removing the tag. Strep-tagged proteins can be isolated in one step from crude cell lysates. Because the Strep-tag elutes under gentle, physiological conditions it is especially suited for generation of functional proteins. The proteins obtained are bioactive and display a very high purity (above 99%).
- Packaging: 10%(v/v), 30-150 μm
SPECIFICATIONS
| Designation | BeaverBeads™ Magrose Strep-Tactin |
| Bead Diameter (µm) | 30μm～150μm |
| Ligand density | ～6mg Strep-Tactin /mL Gel |
| Protein Binding Capacity | ～7mg Strep-tag II protein/mL Gel |
| Slurry Concentration | 10%(v/v) Suspension |
| Storage solution | 1×PBS(0.1%Tween-20+0.05%NaN3) |
| Storage temperature | 2 ~ 8 °C for long; NO FREEZE |
| Binding/Washing Buffer | 10mM Tris-HCl, 150mM NaCl, 1mM EDTA, pH8.0 |
| Elution Buffer | 2.5mM desthiobiotin in Binding Buffer |
| Regeneration Buffer | 0.5M NaOH or 1mM HABA |
FEATURES
1. High capability of antibody binding.
2. Time-saving, simple, and mild.
3. Obtained high-purity protein by one-Step.
4. No need to remove the tag.
